1Infectiouse Diseases Center, Hamadan University of Medical Sciences, Hamadan, Iran.
2Department of Brucellosis, Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization, Karaj, Iran.
3Hamadan Veterinary Council (NGO), Hamadan, Iran.
4Department of Epidemiology & Biostatistics, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran.
5Department of Laboratory Sciences, Central Veterinary Laboratory, Iranian Veterinary Organization (IVO), Hamedan, Ira
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Brucellosis is a zoonotic infectious disease in the western regions of Iran, especially in Hamedan Province. Following the Famenin brucellosis cohort study, the main aim of the current research was the molecular detection of Brucella species in sheep and goats from Famenin, Hamedan Province. A total of 23 Brucella-seropositive samples (sheep=21 and goats=2), which had been screened from 1660 animals in the Famenin cohort study, were used to detect Brucella-DNA using the BCSP31 target gene and IS711 locus. 20 of 23 samples were positive for Brucella infection by using specific primers. Additionally, B. melitensis and B. abortus were confirmed in 90% (n=18) and 10% (n=2) of positive samples, respectively. There was no sample with co-infection of B. abortus and B. melitensis. B. abortus was isolated from one of the goat samples. This is the first report on Brucella species in animals in the region. Brucella melitensis is the dominant species responsible for brucellosis in animals from Famenin. Molecular techniques are reliable tools to detect Brucella infection, especially in cases without serology finding and conclusive results.


Keywords:Animal؛ Molecular detection؛ Brucella abortus؛ Brucella melitensis؛ Famenin