All individuals were invited for interviews and medical examinations to the cohort study center and the field supervisor first familiarized them with the study objectives then they were asked to sign a written consent to start the procedure
Blood samples were drawn after 10-12 hours of fasting.
body analysis is done in durity of fasting time, by inbody 770
Each interview was conducted by means of a structured questionnaire such as demographic ( personal, educational, occupational, and residential details), food frequency, food habits, disease history, family disease history, psychological disorders )such as: anxiety, obsessive-compulsive and depressive disorders
In this stage, trained technicians collected data on the medical history, blood pressure, peripheral pulse and obtained anthropometrical data such as height, weight, hip, and waist sizes Also ECG is taken for them
CBC, FBS, TG, CHOL, HDL, LDL, BUN, Cr, ALT, AST, GGT, ALPH is perform
The storage of specimens such as blood (whole blood, serum, Buffy coat, plasma), hair, nails and urine is also done in the study center.