Authors

Maryam Adabi, Manoochehr Karami, Fariba Keramat,

Mohammad Yousef Alikhani, Somaye Bakhtiari

Affiliations

1: Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
2: Health Sciences Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
3: Department of Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan,
Iran

1: Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

2: Research Center for Health Sciences, Hamadan University of Medical Sciences, Hamadan, Iran

3: Department of Epidemiology, School of Public Health, Hamadan University of Medical

4: Social Determinants of Health Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

5: Modeling of Noncommunicable Diseases Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

Background and Objectives: Brucella is an intracellular pathogen that causes brucellosis in humans and animals. This study aimed to assess the results of brucellosis seroprevalence among participants of the Famenin brucellosis cohort with molecular investigation technique and determine Brucella-approved species.
Materials and Methods: Following the first phase of the Famenin brucellosis cohort in 2016 which investigated the seroprevalence of brucellosis among 2367 participants in Famenin city, a total of 575 people including all seropositive and some seronegative people were examined again by wright serological tests in 2019. The PCR assay was accomplished on all cases that have wright titers ≥ 1/20 for tracing Brucella DNA using BCSP31 target gene and IS711 locus.
Results: Out of 575 studied cases, 145 people had wright titers ≥ 1/20. The PCR reactions of these 145 blood samples were positive in 63/145 (43.44%) tested samples using primers (B4/B5) for Brucella genus detection. In the second PCR assay using specific-primers for Brucella abortus and Brucella melitensis, 18/63 (28.57%) of the samples were diagnosed as B. abortus, and 18/63 (28.57%) were diagnosed as B. melitensis.
Conclusion: In this study, using the selected specific genes for the diagnosis of Brucella in the genus and species levels, the PCR technique was evaluated as a promising method for the rapid and safe detection of brucellosis besides the serological test for more accurate detection of brucellosis especially in cases that are not definitive.